Sato T (IMCR, Gunma Univ.) Iwano T, Kunii M, Harada R, Harada A (Grad. School of Med, Osaka Univ.) Matsuda S, Yuzaki M (Medical school in Keio Univ.) Mizuguchi R, Yoshihara Y (RIKEN Brain Sci.) Jung Y (Medical school in Dongguk Univ.) Hagiwara H (Medical school in Teikyo Univ.)
About
Small GTP-binding protein Rab8 is known to play an essential role in intracellular transport and cilia formation. We generated knockout mice of two Rab8 isoforms Rab8a and Rab8b.Rab8aKO mice exhibited mislocalisation of apical markers and died. Although the Rab8bKO mice did not display an overt phenotype, Rab8a and Rab8b double-knockout (DKO) mice exhibited of apical protein mislocalisation and died about a week earlier than rab8aKO. On the other hand, the morphology and the length of various primary and/or motile cilia in ciliated cells in DKO mice appeared normal. However, an additional knockdown of Rab10 in DKO cells greatly reduced the percentage of ciliated cells. Our results highlight the compensatory effect of Rab8a and Rab8b in apical transport. And simultaneous loss of Rab8a and Rab8b has little effect on ciliogenesis, whereas additional loss of Rab10 greatly affects ciliogenesis.
Paper information
Rab8a and Rab8b are essential for several apical transport pathways but insufficient for ciliogenesis.
Sato T, Iwano T, Kunii M, Matsuda S, Mizuguchi R, Jung Y, Hagiwara H, Yoshihara Y, Yuzaki M, Harada R, Harada A.
Journal of Cell Science 127:422-31, 2014
