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群馬大学 生体調節研究所

アクセス

IMCR Joint Usage/Research Support Center

大橋顔写真

Assistant Professor: Kazuto Ohashi
ohashi*gunma-u.ac.jp
(*=@)
Lab website

 

 

 

Member

Assistant Professor: Kazuto Ohashi
Technical Officer: Masayuki Tobo
Technical Officer: Junki Kohmaru

Research

Kynurenic acid (KA) is a tryptophan metabolite and is produced by kynurenine aminotransferases (KATs). It is known that KA interacts with cell surface receptors (GPR35 and N-methyl-D-aspartate receptor). Altered level of KA is associated with type2 diabetes and depression. Although KA is produced from bacteria to human, its intracellular physiological function and metabolic regulation remain poorly understood.
I aim to elucidate an intracellular function and metabolism of KA. I will study the importance of KA in cell metabolism and physiology using engineered yeast cells deleted for KATs. Also, I will investigate the enzymatic properties of purified KATs to understand the regulation mechanisms of KA synthesis.
Eホームページ図 テニュアトラック大橋

On-going projects

  1. Genetic and biochemical study on physiological roles of KA
  2. Enzymatic study on regulation mechanisms of KATs
  3. Investigation of the toxicity of excess tryptophan

Keywords

kynurenic acid, kynurenine pathway, kynurenine aminotransferase, NAD+, NADP+, NADH, NADPH, yeast, mitochondria

Select References

  1. Kazuto Ohashi, Shigeyuki Kawai, and Kousaku Murata
    Secretion of Quinolinic Acid, an Intermediate in the Kynurenine Pathway, for Utilization in NAD+ Biosynthesis in the Yeast Saccharomyces cerevisiae.
    Eukaryotic Cell, 12, 648-653 (2013)
  2.  Kazuto Ohashi, Romanas Chaleckis, Masak Takaine, Craig E. Wheelock, and Satoshi Yoshida
    Kynurenine aminotransferase activity of Aro8/Aro9 engage tryptophan degradation by producing kynurenic acid in Saccharomyces cerevisiae
    Scientific Reports, 7, 12180 (2017)

 

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