Assistant Professor: Kazuto Ohashi
Technical Officer: Masayuki Tobo
Technical Officer: Junki Kohmaru
Kynurenic acid (KA) is a tryptophan metabolite and is produced by kynurenine aminotransferases (KATs). It is known that KA interacts with cell surface receptors (GPR35 and N-methyl-D-aspartate receptor). Altered level of KA is associated with type2 diabetes and depression. Although KA is produced from bacteria to human, its intracellular physiological function and metabolic regulation remain poorly understood.
I aim to elucidate an intracellular function and metabolism of KA. I will study the importance of KA in cell metabolism and physiology using engineered yeast cells deleted for KATs. Also, I will investigate the enzymatic properties of purified KATs to understand the regulation mechanisms of KA synthesis.
- Genetic and biochemical study on physiological roles of KA
- Enzymatic study on regulation mechanisms of KATs
- Investigation of the toxicity of excess tryptophan
kynurenic acid, kynurenine pathway, kynurenine aminotransferase, NAD+, NADP+, NADH, NADPH, yeast, mitochondria
- Kazuto Ohashi, Shigeyuki Kawai, and Kousaku Murata
Secretion of Quinolinic Acid, an Intermediate in the Kynurenine Pathway, for Utilization in NAD+ Biosynthesis in the Yeast Saccharomyces cerevisiae.
Eukaryotic Cell, 12, 648-653 (2013)
- Kazuto Ohashi, Romanas Chaleckis, Masak Takaine, Craig E. Wheelock, and Satoshi Yoshida
Kynurenine aminotransferase activity of Aro8/Aro9 engage tryptophan degradation by producing kynurenic acid in Saccharomyces cerevisiae
Scientific Reports, 7, 12180 (2017)