Akira Sen1,2#, Atsuki Imai1#, Eiji Miyauchi1, Kota Yanagisawa1, Tsukasa Oda1, Fuki Sasaki1, Shintaro Uchida3, Takuhisa Okada3, Takehiko Yokobori4, Hiroshi Saeki3, Toshitaka Odamaki2, Nobuo Sasaki1* 1: The Laboratory for Mucosal Ecosystem Design, Institute for Molecular and Cellular Regulation, Gunma University. 2: Innovative Research Institute, Morinaga Milk Industry Co Ltd 3: Department of General Surgical Science, Gastroenterological Surgery, Gunma University, Graduate School of Medicine 4: Division of Gene Therapy Science, Gunma University Initiative for Advanced Research, # equally contribution * Corresponding author
About
Previous studies on probiotics have used cell lines, such as Caco-2, derived from colorectal cancer patient. In this study, the research group used organoid culture system that is close to the “normal human intestinal tract”. They succeeded in confirming a more physiological response of the Bifidobacteria in organoid compared to Caco-2. These results demonstrate the usefulness of this groundbreaking evaluation system for more accurately understanding the impact of probiotics and gut microbes on our health. The results of this research are also expected to provide new guidelines for future functional evaluation in the health food and medical field.
Paper information
Sen A, Imai A, Miyauchi E, Yanagisawa K, Oda T, Sasaki F, Uchida S, Okada T, Yokobori T, Saeki H, Odamaki T, Sasaki N. Comprehensive gene expression analysis of organoid-derived healthy human colonic epithelium and cancer cell line stimulated with live probiotic bacteria. Sci Rep. 2025 July; 22325
Online URL
https://www.nature.com/articles/s41598-025-07391-x
